DNA Sequence Alignment during Homologous Recombination
نویسندگان
چکیده
منابع مشابه
DNA Sequence Alignment by Microhomology Sampling during Homologous Recombination
Homologous recombination (HR) mediates the exchange of genetic information between sister or homologous chromatids. During HR, members of the RecA/Rad51 family of recombinases must somehow search through vast quantities of DNA sequence to align and pair single-strand DNA (ssDNA) with a homologous double-strand DNA (dsDNA) template. Here, we use single-molecule imaging to visualize Rad51 as it a...
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DNA sequence alignment is a prerequisite to virtually all comparative genomic analyses, including the identification of conserved sequence motifs, estimation of evolutionary divergence between sequences, and inference of historical relationships among genes and species. While it is mere common sense that inaccuracies in multiple sequence alignments can have detrimental effects on downstream ana...
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The DNA strand exchange protein Rad51 provides a safe mechanism for the repair of DNA breaks using the information of a homologous DNA template. Homologous recombination (HR) also plays a key role in the response to DNA damage that impairs the advance of the replication forks by providing mechanisms to circumvent the lesion and fill in the tracks of single-stranded DNA that are generated during...
متن کاملFunctions of homologous DNA recombination
Homologous DNA (or genetic) recombination plays important roles in heredity and evolution. A recent study has shown that a physical linkage established by recombination between two or more homologous DNA regions or molecules is essential for the precise disjunction of homologous chromosomes in meiosis and the recovery of collapsed DNA replication forks. These physical linkages in recombination ...
متن کاملDNA Assembly Technologies Based on Homologous Recombination
The origins of the recombinant DNA technology can be traced back to the discovery of restriction enzymes and the generation of the first recombinant DNA molecule over 40 years ago (1–3). Since then, and with the emergence of PCR, scientists have generated a number of elegant cloning systems that enable the manipulation of DNA fragments in various ways (for recent reviews see Refs 4–7). Some rem...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2016
ISSN: 0021-9258
DOI: 10.1074/jbc.r116.724807